GMOs

For GMOs the improvements will be two-fold: the DECATHLON project will lead to improved multiplexing (based on PCR derived methodologies), i.e. targeting more events, constructs and/or GMO elements than is currently achievable, and thus to faster testing and significantly improved cost-efficiency. Multiplexing is particularly useful in view of the vast and increasing number of GMOs being brought into commerce around the globe, in order to promote the aptness of food inspection agencies to detect their potential presence in foods, ensuring that consumers’ and producers’ confidence in the authenticity of products will not be harmed. Primarily digital (droplet) PCR (ddPCR) will be evaluated and validated to this effect, but other aspects may be included throughout the project duration as well. Such ddPCR methods will improve the limit of detection and limit and accuracy of quantitation, in particular at lower contamination levels. This will lead to improved analytical reliability. Multiplex event specific ddPCR is expected to circumvent initial element screening as it will permit direct quantitation of all members of a given group of GMOs, e.g. authorised soybean events in the ingredient soybean.