D4.1 Performance and cost effectiveness of ddPCR for detection of GMOs in the EU

The number of authorized GMOs has increased over time and with that the required number of analyses with qPCR has also increased. This increase in number of analyses is making the detection and quantification of GMOs in different samples more and more expensive. To address the issue of cost effectiveness, new multiplex ddPCR methods were developed within the Decathlon project. The performance parameters of the new multiplex methods were evaluated according to the MPPs set in Deliverable D6.1.
Although the majority of the methods were tested on GMOs authorised in the EU, the results on transfer of methods from qPCR to ddPCR and the performance parameters of multiplex ddPCR methods imply that the same system can also be applied to testing for unauthorised GMOs. These developments could serve as a starting point for additional new multiplex ddPCR methods, which would in the end cover the whole spectrum of GMOs.

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